wt 1 Search Results


wt1  (Proteintech)
95
Proteintech wt1
Wt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/pmc09852089__res___132___187___s001-70-46-47?v=Proteintech
Average 95 stars, based on 1 article reviews
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wt 1  (OriGene)
94
OriGene wt 1
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
Wt 1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/pmc12823855-3-0-12?v=OriGene
Average 94 stars, based on 1 article reviews
wt 1 - by Bioz Stars, 2026-07
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60188 1 ig  (Proteintech)
96
Proteintech 60188 1 ig
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
60188 1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/pmc12953771-39-5-3?v=Proteintech
Average 96 stars, based on 1 article reviews
60188 1 ig - by Bioz Stars, 2026-07
96/100 stars
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rabbit anti wt1  (Aviva Systems)
90
Aviva Systems rabbit anti wt1
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
Rabbit Anti Wt1, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/pmc06175865__41598_2018_33256_MOESM1_ESM-18-6-9?v=Aviva+Systems
Average 90 stars, based on 1 article reviews
rabbit anti wt1 - by Bioz Stars, 2026-07
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anti wt1 antibody c 19  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti wt1 antibody c 19
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
Anti Wt1 Antibody C 19, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/us08299234-179-9-12?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
anti wt1 antibody c 19 - by Bioz Stars, 2026-07
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antibody anti wt1  (R&D Systems)
93
R&D Systems antibody anti wt1
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
Antibody Anti Wt1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/10__7554_slash_elife__103783__3-320-20-25?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
antibody anti wt1 - by Bioz Stars, 2026-07
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pcmv sp2  (OriGene)
90
OriGene pcmv sp2
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
Pcmv Sp2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/pm33234712-259-25-31?v=OriGene
Average 90 stars, based on 1 article reviews
pcmv sp2 - by Bioz Stars, 2026-07
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wt1 cre col7a11flox flox mice  (Cyagen Biosciences)
93
Cyagen Biosciences wt1 cre col7a11flox flox mice
Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) <t>,</t> <t>WT-1</t> (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).
Wt1 Cre Col7a11flox Flox Mice, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/10__1172_slash_jci188822-246-3-17?v=Cyagen+Biosciences
Average 93 stars, based on 1 article reviews
wt1 cre col7a11flox flox mice - by Bioz Stars, 2026-07
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mouse anti wt1 monoclonal antibody  (Novus Biologicals)
93
Novus Biologicals mouse anti wt1 monoclonal antibody
Figure 1. Generation of podocyte-specific Pfn1-KO mice results in severe proteinuria and kidney failure. (A) Representative immunoblot images of profilin1 expression in primary podocytes freshly isolated from littermate control (Ctrl) and Pfn1-KO mice (age P7). (B) Representative immunofluorescence images of profilin1 (green) and <t>WT1</t> (red) in control and Pfn1-KO primary podocytes. Scale bar: 10 μm. (C) Representative immunofluorescence images of profilin1 (green) and nephrin (red) on kidney sections of control and Pfn1-KO mice (age 3 weeks). Scale bars: 10 μm. (D) Pfn1-KO mice (red) failed to gain body weight by 8 weeks of age compared with control mice (green). n = 9 mice. *P < 0.05 vs. control. (E) The survival curve of Pfn1-KO mice (red) demon- strates approximately 90% death by 12 weeks of age. n = 8 mice. (F) SDS-PAGE (Coomassie blue staining) of standard BSA and of urine samples from Pfn1-KO mice at 4 weeks of age demonstrates albuminuria. Equal volumes of standard BSA and urine (4 μL) were loaded in each lane. (G) Quantification of urine albumin normalized to creatinine at 2, 6, and 10 weeks of age. n = 6 mice. *P < 0.05 vs. control. (H) Elevated plasma creatinine in Pfn1-KO mice at 3, 6, and 10 weeks of age. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.
Mouse Anti Wt1 Monoclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/10__1172_slash_jci171237-186-43-47?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
mouse anti wt1 monoclonal antibody - by Bioz Stars, 2026-07
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monoclonal unconjugated antibody wt1  (Novus Biologicals)
92
Novus Biologicals monoclonal unconjugated antibody wt1
Characterization of NPCs colonies for <t>WT1</t> by immunofluorescence staining as performed on three different amniotic fluid derived NPCs. This figure shows immunofluorescence analysis for positive nuclear marker WT1 (a) Bright field image of NPCs at 40x showed irregular with ill-defined borders having tightly packed cells for the three different amniotic fluid derived NPCs. (b) Counter DAPI nuclear stain showed blue color of Nucleus. (c) Characteristic of NPCs, WT1 is shown in nuclear compartment of cells.
Monoclonal Unconjugated Antibody Wt1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/pmc09378384-43-71-79?v=Novus+Biologicals
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monoclonal unconjugated antibody wt1 - by Bioz Stars, 2026-07
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rabbit anti wt1 antibody  (Novus Biologicals)
93
Novus Biologicals rabbit anti wt1 antibody
Characterization of NPCs colonies for <t>WT1</t> by immunofluorescence staining as performed on three different amniotic fluid derived NPCs. This figure shows immunofluorescence analysis for positive nuclear marker WT1 (a) Bright field image of NPCs at 40x showed irregular with ill-defined borders having tightly packed cells for the three different amniotic fluid derived NPCs. (b) Counter DAPI nuclear stain showed blue color of Nucleus. (c) Characteristic of NPCs, WT1 is shown in nuclear compartment of cells.
Rabbit Anti Wt1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/10__1681_slash_asn__2019121261-124-21-26?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
rabbit anti wt1 antibody - by Bioz Stars, 2026-07
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rabbit anti wilms tumor protein  (Novus Biologicals)
91
Novus Biologicals rabbit anti wilms tumor protein
Characterization of NPCs colonies for <t>WT1</t> by immunofluorescence staining as performed on three different amniotic fluid derived NPCs. This figure shows immunofluorescence analysis for positive nuclear marker WT1 (a) Bright field image of NPCs at 40x showed irregular with ill-defined borders having tightly packed cells for the three different amniotic fluid derived NPCs. (b) Counter DAPI nuclear stain showed blue color of Nucleus. (c) Characteristic of NPCs, WT1 is shown in nuclear compartment of cells.
Rabbit Anti Wilms Tumor Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wt+1/10__1681_slash_asn__2017010107-118-39-63?v=Novus+Biologicals
Average 91 stars, based on 1 article reviews
rabbit anti wilms tumor protein - by Bioz Stars, 2026-07
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Image Search Results


Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) , WT-1 (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).

Journal: Frontiers in Oncology

Article Title: Primary extraovarian adult granulosa cell tumor of the greater omentum: a case report and literature review

doi: 10.3389/fonc.2025.1689815

Figure Lengend Snippet: Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) , WT-1 (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).

Article Snippet: WT-1 , Positive , Strong,diffuse nucleus , Supports diagnosis , OTIRIH(Rabbit Monoclonal), Zhongshan Golden Bridge.

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry

Figure 1. Generation of podocyte-specific Pfn1-KO mice results in severe proteinuria and kidney failure. (A) Representative immunoblot images of profilin1 expression in primary podocytes freshly isolated from littermate control (Ctrl) and Pfn1-KO mice (age P7). (B) Representative immunofluorescence images of profilin1 (green) and WT1 (red) in control and Pfn1-KO primary podocytes. Scale bar: 10 μm. (C) Representative immunofluorescence images of profilin1 (green) and nephrin (red) on kidney sections of control and Pfn1-KO mice (age 3 weeks). Scale bars: 10 μm. (D) Pfn1-KO mice (red) failed to gain body weight by 8 weeks of age compared with control mice (green). n = 9 mice. *P < 0.05 vs. control. (E) The survival curve of Pfn1-KO mice (red) demon- strates approximately 90% death by 12 weeks of age. n = 8 mice. (F) SDS-PAGE (Coomassie blue staining) of standard BSA and of urine samples from Pfn1-KO mice at 4 weeks of age demonstrates albuminuria. Equal volumes of standard BSA and urine (4 μL) were loaded in each lane. (G) Quantification of urine albumin normalized to creatinine at 2, 6, and 10 weeks of age. n = 6 mice. *P < 0.05 vs. control. (H) Elevated plasma creatinine in Pfn1-KO mice at 3, 6, and 10 weeks of age. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Journal: Journal of Clinical Investigation

Article Title: Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/jci171237

Figure Lengend Snippet: Figure 1. Generation of podocyte-specific Pfn1-KO mice results in severe proteinuria and kidney failure. (A) Representative immunoblot images of profilin1 expression in primary podocytes freshly isolated from littermate control (Ctrl) and Pfn1-KO mice (age P7). (B) Representative immunofluorescence images of profilin1 (green) and WT1 (red) in control and Pfn1-KO primary podocytes. Scale bar: 10 μm. (C) Representative immunofluorescence images of profilin1 (green) and nephrin (red) on kidney sections of control and Pfn1-KO mice (age 3 weeks). Scale bars: 10 μm. (D) Pfn1-KO mice (red) failed to gain body weight by 8 weeks of age compared with control mice (green). n = 9 mice. *P < 0.05 vs. control. (E) The survival curve of Pfn1-KO mice (red) demon- strates approximately 90% death by 12 weeks of age. n = 8 mice. (F) SDS-PAGE (Coomassie blue staining) of standard BSA and of urine samples from Pfn1-KO mice at 4 weeks of age demonstrates albuminuria. Equal volumes of standard BSA and urine (4 μL) were loaded in each lane. (G) Quantification of urine albumin normalized to creatinine at 2, 6, and 10 weeks of age. n = 6 mice. *P < 0.05 vs. control. (H) Elevated plasma creatinine in Pfn1-KO mice at 3, 6, and 10 weeks of age. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Article Snippet: Rabbit anti-profilin1 monoclonal antibody (used for immunofluorescence, Thermo Fisher Scientific, catalog MA5-32683); rabbit anti-profilin1 polyclonal antibody (used for Western blotting, Cell Signaling Technology, catalog 3237S); guinea pig anti-nephrin polyclonal antibody (Progen, catalog GP-N2); rabbit anti-Wilms tumor 1 (WT1) monoclonal antibody (Abcam, catalog ab89901); mouse anti-WT1 monoclonal antibody (Novus Biologicals, catalog NB11-60011); rabbit anti-p21 monoclonal antibody (Abcam, catalog ab188224); mouse anti-p53 monoclonal antibody (Cell Signaling Technology, catalog 2524S); rabbit anti-cyclin monoclonal D1 antibody (Cell Signaling Technology, catalog 2978S); mouse anti-cyclin polyclonal B1 antibody (Cell Signaling Technology, catalog 4138S); mouse anti–Ser 319–phosphorylated γH2AX monoclonal antibody (EMD Millipore, catalog 05-636); rat anti-mCherry monoclonal antibody (Invitrogen, catalog M11217); goat anti-mVenus polyclonal antibody (MyBioSource, catalog MBS448126); Hoechst (Thermo Fisher Scientific, catalog 62249); rabbit anti-GAPDH monoclonal antibody (Cell Signaling Technology, catalog 2118S); mouse anti-GFP monoclonal antibody (Roche, catalog 11814460001); Alexa Fluor 488–conjugated phalloidin (Invitrogen, catalog A12379); Alexa Fluor 594–conjugated phalloidin (Invitrogen, catalog A12381); Alexa Fluor 488–conjugated tubulin (Abcam, catalog ab195883); Alexa Fluor 488 goat anti-mouse IgG antibody (Invitrogen, catalog A11029); Alexa Fluor 488 goat anti-rabbit IgG antibody (Invitrogen, catalog A11034); Alexa Fluor 488 donkey anti-goat IgG antibody (Invitrogen, catalog A11055); Alexa Fluor 594 goat anti-rabbit IgG antibody (Invitrogen, catalog A21207); Alexa Fluor 594 goat anti-mouse (Invitrogen, catalog A11032); Alexa Fluor 594 goat anti-guinea pig (Invitrogen, catalog A11076); Alexa Fluor 594 goat rat (Invitrogen, catalog A11007); Alexa Fluor 647 goat anti-rabbit (Invitrogen, catalog A21245); mouse anti– mouse IgG HRP-conjugated antibody (Rockland, catalog 18-8817- 31); and rabbit anti–mouse IgG HRP-conjugated antibody (Millipore Sigma, catalog AP160P) were purchased commercially.

Techniques: Western Blot, Expressing, Isolation, Control, Immunofluorescence, SDS Page, Staining, Clinical Proteomics

Figure 3. Loss of podocyte Pfn1 results in morphologic MC appearance, chromosomal instability, and dsDNA damage. (A) Representative images of transmission electron micrography demonstrate abnormal MC podocytes in Pfn1-KO mice (arrow) compared with control glomeruli at 4 weeks of age. Scale bar: 1 μm. (B) Immunofluorescence images of primary podocytes isolated from control and Pfn1-KO mice at P7 stained with WT1 (red, podocyte marker) and Hoechst (blue, DNA marker) showing abnormal MC podocytes in Pfn1-KO mice (arrow) compared with control podocytes. Scale bar: 20 μm. (C) Immunofluorescence images of primary culture podocytes isolated from control and Pfn1-KO mice stained with Hoechst and anti-tubulin antibody showing the chromosome bridge (arrow) in a MC Pfn1-KO podocyte. Scale bar: 20 μm. (D) Quantification of the percentage of MC podocytes per field of view in B. Total of 100 fields of view in 5 independent experiments. (E) Quantification of the percentage of chromosome bridge per field of view in C. n = 5 independent experiments. *P < 0.05 vs. control. (F) Immunofluorescence images of primary culture podocytes stained with γ-H2AX (green, double-strand breaks [DSBs] marker) and WT1 (red) showing abnormal MC podocytes in Pfn1-KO mice, as indicated by the arrows. Scale bar: 20 μm. (G) Quantification of γH2AX foci per podocyte nucleus (left) and per podocyte nuclear area (right) in F. Total of 400 cells in 5 independent experiments. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Journal: Journal of Clinical Investigation

Article Title: Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/jci171237

Figure Lengend Snippet: Figure 3. Loss of podocyte Pfn1 results in morphologic MC appearance, chromosomal instability, and dsDNA damage. (A) Representative images of transmission electron micrography demonstrate abnormal MC podocytes in Pfn1-KO mice (arrow) compared with control glomeruli at 4 weeks of age. Scale bar: 1 μm. (B) Immunofluorescence images of primary podocytes isolated from control and Pfn1-KO mice at P7 stained with WT1 (red, podocyte marker) and Hoechst (blue, DNA marker) showing abnormal MC podocytes in Pfn1-KO mice (arrow) compared with control podocytes. Scale bar: 20 μm. (C) Immunofluorescence images of primary culture podocytes isolated from control and Pfn1-KO mice stained with Hoechst and anti-tubulin antibody showing the chromosome bridge (arrow) in a MC Pfn1-KO podocyte. Scale bar: 20 μm. (D) Quantification of the percentage of MC podocytes per field of view in B. Total of 100 fields of view in 5 independent experiments. (E) Quantification of the percentage of chromosome bridge per field of view in C. n = 5 independent experiments. *P < 0.05 vs. control. (F) Immunofluorescence images of primary culture podocytes stained with γ-H2AX (green, double-strand breaks [DSBs] marker) and WT1 (red) showing abnormal MC podocytes in Pfn1-KO mice, as indicated by the arrows. Scale bar: 20 μm. (G) Quantification of γH2AX foci per podocyte nucleus (left) and per podocyte nuclear area (right) in F. Total of 400 cells in 5 independent experiments. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Article Snippet: Rabbit anti-profilin1 monoclonal antibody (used for immunofluorescence, Thermo Fisher Scientific, catalog MA5-32683); rabbit anti-profilin1 polyclonal antibody (used for Western blotting, Cell Signaling Technology, catalog 3237S); guinea pig anti-nephrin polyclonal antibody (Progen, catalog GP-N2); rabbit anti-Wilms tumor 1 (WT1) monoclonal antibody (Abcam, catalog ab89901); mouse anti-WT1 monoclonal antibody (Novus Biologicals, catalog NB11-60011); rabbit anti-p21 monoclonal antibody (Abcam, catalog ab188224); mouse anti-p53 monoclonal antibody (Cell Signaling Technology, catalog 2524S); rabbit anti-cyclin monoclonal D1 antibody (Cell Signaling Technology, catalog 2978S); mouse anti-cyclin polyclonal B1 antibody (Cell Signaling Technology, catalog 4138S); mouse anti–Ser 319–phosphorylated γH2AX monoclonal antibody (EMD Millipore, catalog 05-636); rat anti-mCherry monoclonal antibody (Invitrogen, catalog M11217); goat anti-mVenus polyclonal antibody (MyBioSource, catalog MBS448126); Hoechst (Thermo Fisher Scientific, catalog 62249); rabbit anti-GAPDH monoclonal antibody (Cell Signaling Technology, catalog 2118S); mouse anti-GFP monoclonal antibody (Roche, catalog 11814460001); Alexa Fluor 488–conjugated phalloidin (Invitrogen, catalog A12379); Alexa Fluor 594–conjugated phalloidin (Invitrogen, catalog A12381); Alexa Fluor 488–conjugated tubulin (Abcam, catalog ab195883); Alexa Fluor 488 goat anti-mouse IgG antibody (Invitrogen, catalog A11029); Alexa Fluor 488 goat anti-rabbit IgG antibody (Invitrogen, catalog A11034); Alexa Fluor 488 donkey anti-goat IgG antibody (Invitrogen, catalog A11055); Alexa Fluor 594 goat anti-rabbit IgG antibody (Invitrogen, catalog A21207); Alexa Fluor 594 goat anti-mouse (Invitrogen, catalog A11032); Alexa Fluor 594 goat anti-guinea pig (Invitrogen, catalog A11076); Alexa Fluor 594 goat rat (Invitrogen, catalog A11007); Alexa Fluor 647 goat anti-rabbit (Invitrogen, catalog A21245); mouse anti– mouse IgG HRP-conjugated antibody (Rockland, catalog 18-8817- 31); and rabbit anti–mouse IgG HRP-conjugated antibody (Millipore Sigma, catalog AP160P) were purchased commercially.

Techniques: Transmission Assay, Control, Immunofluorescence, Isolation, Staining, Marker

Figure 5. Loss of podocyte Pfn1 results in podocytopenia and cell cycle entry. (A) A schematic representing cell cycle phase visualization by different colors in podocytes from the R26Fucci2aR Pfn1-KO mice. G1 phase nuclei (mCherry, red); S phase nuclei (both mCherry and mVenus, yellow); and G2 phase nuclei (mVenus, green). (B) Representative immunofluorescence images of podocytes in control-FUCCI and Pfn1-KO-FUCCI glomeruli at 4 weeks of age stained with Cherry (red), Venus (green), and WT1 (blue). Scale bar: 20 μm. (C) Quantification of the distribution of podocytes in cell cycle phase in B. Total of 100 glomeruli in 5 different mice. (D) Primary podocytes isolated from control and Pfn1-KO mice demonstrated a significant decrease in adhesion after plating for 5 minutes and 10 minutes on the collagen type I–coated plates. n = 6 independent experiments. (E) Representative immunofluorescence images of podocytes in control and Pfn1-KO glomeruli at 7 weeks of age stained with WT1 (red). Scale bar: 20 μm. (F) Quantification of podocyte density in glomeruli in E. Total of 40 glomeruli from 5 different mice. (G) Representative immunofluorescence images of urinary podocytes from control and Pfn1-KO mice at 4 weeks of age stained with mCherry (red), mVenus (green), and WT1 (blue). G1 phase podocyte nuclei (red arrow), S phase podocyte nuclei (yellow arrow), and G2 phase podocyte nuclei (green arrow) were depicted. Scale bar: 20 μm. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Journal: Journal of Clinical Investigation

Article Title: Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/jci171237

Figure Lengend Snippet: Figure 5. Loss of podocyte Pfn1 results in podocytopenia and cell cycle entry. (A) A schematic representing cell cycle phase visualization by different colors in podocytes from the R26Fucci2aR Pfn1-KO mice. G1 phase nuclei (mCherry, red); S phase nuclei (both mCherry and mVenus, yellow); and G2 phase nuclei (mVenus, green). (B) Representative immunofluorescence images of podocytes in control-FUCCI and Pfn1-KO-FUCCI glomeruli at 4 weeks of age stained with Cherry (red), Venus (green), and WT1 (blue). Scale bar: 20 μm. (C) Quantification of the distribution of podocytes in cell cycle phase in B. Total of 100 glomeruli in 5 different mice. (D) Primary podocytes isolated from control and Pfn1-KO mice demonstrated a significant decrease in adhesion after plating for 5 minutes and 10 minutes on the collagen type I–coated plates. n = 6 independent experiments. (E) Representative immunofluorescence images of podocytes in control and Pfn1-KO glomeruli at 7 weeks of age stained with WT1 (red). Scale bar: 20 μm. (F) Quantification of podocyte density in glomeruli in E. Total of 40 glomeruli from 5 different mice. (G) Representative immunofluorescence images of urinary podocytes from control and Pfn1-KO mice at 4 weeks of age stained with mCherry (red), mVenus (green), and WT1 (blue). G1 phase podocyte nuclei (red arrow), S phase podocyte nuclei (yellow arrow), and G2 phase podocyte nuclei (green arrow) were depicted. Scale bar: 20 μm. *P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Article Snippet: Rabbit anti-profilin1 monoclonal antibody (used for immunofluorescence, Thermo Fisher Scientific, catalog MA5-32683); rabbit anti-profilin1 polyclonal antibody (used for Western blotting, Cell Signaling Technology, catalog 3237S); guinea pig anti-nephrin polyclonal antibody (Progen, catalog GP-N2); rabbit anti-Wilms tumor 1 (WT1) monoclonal antibody (Abcam, catalog ab89901); mouse anti-WT1 monoclonal antibody (Novus Biologicals, catalog NB11-60011); rabbit anti-p21 monoclonal antibody (Abcam, catalog ab188224); mouse anti-p53 monoclonal antibody (Cell Signaling Technology, catalog 2524S); rabbit anti-cyclin monoclonal D1 antibody (Cell Signaling Technology, catalog 2978S); mouse anti-cyclin polyclonal B1 antibody (Cell Signaling Technology, catalog 4138S); mouse anti–Ser 319–phosphorylated γH2AX monoclonal antibody (EMD Millipore, catalog 05-636); rat anti-mCherry monoclonal antibody (Invitrogen, catalog M11217); goat anti-mVenus polyclonal antibody (MyBioSource, catalog MBS448126); Hoechst (Thermo Fisher Scientific, catalog 62249); rabbit anti-GAPDH monoclonal antibody (Cell Signaling Technology, catalog 2118S); mouse anti-GFP monoclonal antibody (Roche, catalog 11814460001); Alexa Fluor 488–conjugated phalloidin (Invitrogen, catalog A12379); Alexa Fluor 594–conjugated phalloidin (Invitrogen, catalog A12381); Alexa Fluor 488–conjugated tubulin (Abcam, catalog ab195883); Alexa Fluor 488 goat anti-mouse IgG antibody (Invitrogen, catalog A11029); Alexa Fluor 488 goat anti-rabbit IgG antibody (Invitrogen, catalog A11034); Alexa Fluor 488 donkey anti-goat IgG antibody (Invitrogen, catalog A11055); Alexa Fluor 594 goat anti-rabbit IgG antibody (Invitrogen, catalog A21207); Alexa Fluor 594 goat anti-mouse (Invitrogen, catalog A11032); Alexa Fluor 594 goat anti-guinea pig (Invitrogen, catalog A11076); Alexa Fluor 594 goat rat (Invitrogen, catalog A11007); Alexa Fluor 647 goat anti-rabbit (Invitrogen, catalog A21245); mouse anti– mouse IgG HRP-conjugated antibody (Rockland, catalog 18-8817- 31); and rabbit anti–mouse IgG HRP-conjugated antibody (Millipore Sigma, catalog AP160P) were purchased commercially.

Techniques: Immunofluorescence, Control, Staining, Isolation

Characterization of NPCs colonies for WT1 by immunofluorescence staining as performed on three different amniotic fluid derived NPCs. This figure shows immunofluorescence analysis for positive nuclear marker WT1 (a) Bright field image of NPCs at 40x showed irregular with ill-defined borders having tightly packed cells for the three different amniotic fluid derived NPCs. (b) Counter DAPI nuclear stain showed blue color of Nucleus. (c) Characteristic of NPCs, WT1 is shown in nuclear compartment of cells.

Journal: Pakistan Journal of Medical Sciences

Article Title: Differentiation of CD117 + Amniotic Fluid Stem Cells towards Nephron Progenitors

doi: 10.12669/pjms.38.6.4887

Figure Lengend Snippet: Characterization of NPCs colonies for WT1 by immunofluorescence staining as performed on three different amniotic fluid derived NPCs. This figure shows immunofluorescence analysis for positive nuclear marker WT1 (a) Bright field image of NPCs at 40x showed irregular with ill-defined borders having tightly packed cells for the three different amniotic fluid derived NPCs. (b) Counter DAPI nuclear stain showed blue color of Nucleus. (c) Characteristic of NPCs, WT1 is shown in nuclear compartment of cells.

Article Snippet: The amniotic fluid samples were washed thoroughly with Phosphate Buffered Saline then fixed at 1:1 acetone: methanol for twenty minutes at 25°C, after washing with Buffer Saline containing tween 20 (PBST) three times Triton X-100 was use for permeabilization of cell membrane then again washed with PBST, it was clogged by bovine serum albumin (BSA) 1% plus PBS (without Calcium &Magnesium) for 30 minutes at Room Temperature and incubated with primary monoclonal unconjugated antibody WT1 (WT1/857 + 6F-H2 clone, Novus Biologicals, Biotechne, USA) as positive and CD117 (CD117 APC, Thermofisher, 104D2) as a negative control for an hour.

Techniques: Immunofluorescence, Staining, Derivative Assay, Marker